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6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway Anais da ABC (AABC)
ZHAO,XIAO-E; YANG,ZHENSHAN; GAO,ZHEN; GE,JUNBANG; WEI,QIANG; MA,BAOHUA.
This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone mesenchymal stem cells; Bromoindirubin oxime; Osteogenetic differentiation; Canine.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100610
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A comparison of the immunological effects of propofol and isoflurane for maintenance of anesthesia in healthy dogs OAK
TOMIHARI, Mizuki; NISHIHARA, Akira; SHIMADA, Terumasa; YANAGAWA, Masashi; MIYOSHI, Masafumi; MIYAHARA, Kazurou; OISHI, Akihiro; 富張, 瑞樹; 宮原, 和郎; 大西, 明弘.
Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clinical blood hematology and immunological profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy adult beagles were included in this study, randomly assigned to the propofol anesthesia group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups, the number of lymphocytes in peripheral blood decreased after 2...
Palavras-chave: Anesthesia; Canine; Immuno-suppression; Isoflurane; Propofol.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4209
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A rare retrobulbar and chiasmal meningioma in a dog Ciência Rural
Aiello,Graciane; Ripplinger,Angel; Schwab,Marcelo Luís; Ferrarin,Dênis Antonio; Rauber,Júlia da Silva; Areco,Walter Cardozo; Flores,Mariana Martins; Mazzanti,Alexandre.
ABSTRACT: A 14-year-old female Dachshund was referred to a veterinary hospital with a history and signalment of head pressing, compulsive walking, and right circling. At ophthalmologic examination, a mature cataract and absence of photomotor reflex in the right eye were observed. The neurological exam revealed multifocal encephalic signs. At necropsy, a conical solid tan mass was observed involving the right optic nerve throughout its extension to the optic chiasm. Histopathological findings confirmed a retrobulbar papillary meningioma, considered rare and seldom included as differential diagnosis in patients with neurological signs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ocular neoplasm; Papillary meningioma; Central nervous system; Canine.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700551
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Acoustic radiation force impulse (ARFI) elastography of adrenal glands in healthy adult dogs Arq. Bras. Med. Vet. Zootec.
Fernandez,S.; Feliciano,M.A.R.; Borin-Crivellenti,S.; Crivellenti,L.Z.; Maronezi,M.C.; Simões,A.P.R.; Silva,P.D.A.; Uscategui,R.R.; Cruz,N.R.N.; Santana,A.E.; Vicente,W.R.R..
ABSTRACT To describe the ARFI elastography in the evaluation of the adrenal glands in adult dogs, to evaluate the stiffness of adrenal parenchyma and to determine the qualitative and quantitative standards of the ARFI technique, which have not yet been described in veterinary medicine. Sixty adrenal glands from thirty healthy adult dogs were evaluated by B-mode and qualitative and quantitative ARFI elastography to assess the stiffness of healthy parenchyma and to determine the quality and quantity (shear velocity) standards of ARFI technique in body, cranial and caudal adrenal poles. Findings of ultrasonography evaluations were normal. Qualitative elastography showed that adrenal glands were not deformable and presented homogeneous middle-gray areas. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Adrenal; Ultrasound.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200340
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Acoustic radiation force impulse (ARFI) elastography of testicular disorders in dogs: preliminary results Arq. Bras. Med. Vet. Zootec.
Feliciano,M.A.R.; Maronezi,M.C.; Simões,A.P.R.; Maciel,G.S.; Pavan,L.; Gasser,B.; Silva,P.; Uscategui,R.R.; Carvalho,C.F.; Canola,J.C.; Vicente,W.R.R..
The aim of this study was to describe the use of acoustic radiation force impulse (ARFI) elastography in the evaluation of testicular disorders in dogs. Eighteen dogs with testicular disorders (thirty-six testicles) were assessed. Echotexture, size, contours and margins of testes were analysed by ultrasonography. Deformities and tissue stiffness (greyscale and homogenous or heterogeneous) were evaluated by qualitative elastography and shear velocity was determined quantitatively. Subsequent to orchiectomy, testicular samples were collected for histopathology analysis and thirty-six disorders were identified. Qualitative elastography revealed that normal healthy testicular tissues were homogenous and not pliable while the affected testicles had alterations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Stiffness; Testicular disease.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200283
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Acoustic radiation force impulse elastography of the eyes of brachycephalic dogs Arq. Bras. Med. Vet. Zootec.
Abreu,T.G.M.; Feliciano,M.A.R.; Renzo,R.; Kobashigawa,K.K.; Chacaltana,F.D.Y.C.; Crivelaro,R.M.; Silveira,C.P. B.; Cruz,N.R.N.; Aldrovani,M.; Maronezi,M.C.; Silva,P.A.; Thiesen,R.; Laus,J.L..
ABSTRACT Eyes from brachycephalic dogs were studied by acoustic radiation force impulse (ARFI) elastography to establish quantitative and qualitative reference values related to the shape and rigidity of some ocular structures. Eighty-four eyes from 42 adult brachycephalic dogs were included in this study. Animals were subjected to physical, laboratory, and ophthalmic examinations. Following confirmation of good health, the dogs were subjected to ultrasonography and ARFI elastography, emphasizing the optic nerve, ciliary body, and lens. On qualitative elastography, homogeneous characteristics were observed on the ciliary body and optic nerve. Aqueous and vitreous humors were visualized as mosaic-like images. Quantitative elastography showed the mean optic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Ocular structures; Ultrasound.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401045
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Acute-on-chronic subdural cranial hematoma in a dog: case report Arq. Bras. Med. Vet. Zootec.
Bonelli,M.A.; Silva,I.C.C.; Fernandes,T.H.T.; Schwarz,T.; Costa,F.S..
ABSTRACT A ten-month-old male Yorkshire terrier dog was evaluated via CT four months after traumatic brain injury. The head CT scan showed dilated ventricles associated with a peripheral crescent-shaped collection of blood near the right parietal bone with a mineralized area. The visceral layer of the hematoma was hyperattenuating on the native scan and showed moderate contrast enhancement after administration of intravenous iodinated contrast medium. No fractures were identified in the calvarium. These findings were compatible with acute-on-chronic calcified subdural hematoma, which have been described with more detail in humans. This is the first report to include the description of the imaging findings related to this condition using computed tomography...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Head trauma; Traumatic brain injury; Computed tomography.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100131
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Antibodies against rabies virus in dogs with and without history of vaccination in Santa Maria - RS - Brazil Ciência Rural
Fernandes,Karina Gonzalez; Martins,Mathias; Amaral,Bruna Portolan; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Flores,Eduardo Furtado.
ABSTRACT: The present study investigated the frequency and magnitude of neutralizing antibodies to rabies virus (RABV) in dogs with and without historic of vaccination in Santa Maria/RS. Group A included serum samples from 440 dogs with recent historic of vaccination against rabies, obtained during the 2015 rabies vaccination campaign. Group B included 300 serum samples from dogs submitted to the Veterinary Hospital of the Universidade Federal de Santa Maria in 2015, whose historic of rabies vaccination was unknown. Serum samples were submitted to the rapid fluorescent focus inhibition test (RFFIT) to detect neutralizing antibodies against RABV. In group A, 70.6% (310/440) of the samples had neutralizing antibody titers ≥0.5 international units per...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Immunity; RABV; Serological response; Vaccine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100452
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Aortic thrombosis in a dog with chronic lymphocytic leukemia Ciência Rural
Ripplinger,Angel; Silva,Ana Paula da; Wrzesinski,Mathias Reginatto; Ferrarin,Dênis Antonio; Schwab,Marcelo Luís; Pozzobon,Ricardo; Flores,Mariana Martins; Thompson,Ruth Pamela; Mario,Fabiana; Mazzanti,Alexandre.
ABSTRACT: The aim of this report was to describe a case of aortic thrombosis (AT) secondary to chronic lymphocytic leukemia (CLL). Although, different types of neoplasms are described as possible causes of aortic thrombosis, CLL was not yet considered. The dog showed signs of lameness that worsened with exercise. The diagnosis of AT was made by ultrasound examination. The diagnosis of CLL was made by necropsy, which showed the presence of small lymphocytes with the appearance of mature lymphocytes in the bone marrow, spleen, liver and kidneys. The importance of including CLL in the possible causes of AT in dogs, in addition to the suspicion of AT in cases of neuromuscular disease, was highlighted.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Lymphoid leukemia; Ischemic neuromyopathy; Thrombus.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400601
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Artificial Insemination with Canine Spermatozoa Frozen in a Skim Milk/Glucose-Based Extender OAK
ABE, Yasuyuki; LEE, Dong-Soo; SANO, Hikaru; AKIYAMA, Koji; YANAGIMOTO-UETA, Yoshiko; ASANO, Tomoyoshi; SUWA, Yoshinori; SUZUKI, Hiroshi; 鈴木, 宏志.
Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-thawed in the presence of glycerol was examined. There was a tendency for the proportion of motile spermatozoa exposed to SG-based extender for 3 h to be higher than that exposed for 1 h, but the difference was not significant. The motility and other viability parameters of canine spermatozoa after thawing were similar to those obtained with an egg yolk-based...
Palavras-chave: Canine; Cryopreservation; Skim milk/glucose-based extender; Spermatozoa.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3030
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Astasia and pyrexia related to Borrelia garinii infection in two dogs in Hokkaido, Japan. OAK
INOKUMA, Hisashi; MAETANI, Shigeki; FUJITSUKA, Jyunji; TAKANO, Ai; SATO, Kozue; FUKUI, Takashi; MASUZAWA, Toshiyuki; KAWABATA, Hiroki; 猪熊, 壽.
Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs was Lyme disease related to B. garinii infection.
Palavras-chave: Borrelia garinii; Canine; Japan; Lyme disease.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4099
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Autologous bone marrow transplantation in a dog with lymphoma: a clinical study Ciência Rural
Cápua,Maria Luísa Buffo de; Coleta,Flávia Eiras Dela; Miotto,Mariana Rodrigues; Calazans,Sabryna Gouveia; Godoy,Aline Vieira; Nakage,Ana Paula Massae; Lisbão,Carolina Bonduki Sales; Daleck,Carlos Roberto; Santana,Aureo Evangelista.
The objective of this study is to provide the first report of bone marrow transplantation (BMT) in dogs in Brazil. A Rottweiler with cutaneous lymphoma was submitted to a twelve-week Madison-Wisconsin chemotherapy protocol followed by autologous bone marrow transplantation. For this, 10mL kg-1 of bone marrow was collected simultaneously from both iliac crests and cryopreserved in a freezer at -80°C. The conditioning step was performed by administering cyclophosphamide by intravenous route at 400mg m-2. Bone marrow was reinfused after defrosting in a water bath at 37°C. Bone marrow nucleated cell counts before and after freezing, showed a small relative loss of nucleated cells (35.10 and 31.80x10³µL-1 , respectively). Cyclophosphamide induced neutropenia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Cutaneous lymphoma; Autologous bone marrow transplant; Chemotherapy.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000200045
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Babesia gibsoni Infections in Dogs OAK
Yamane, Itsuro; Conrad, Patricia A.; Gardner, Ian.
Palavras-chave: Babesia gibsoni; Canine; Dogs; Anemia; Babesiosis.
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/194
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B-mode and Doppler ultrasonography of adrenal glands of healthy dogs Arq. Bras. Med. Vet. Zootec.
Fernandez,S.; Feliciano,M.A.R.; Crivellenti,S.B.; Crivellenti,L.Z.; Simões,A.P.R.; Maronezi,M.C.; Uscategui,R.R.; Capela,C.R.; Gilor,C.; Santana,A.E.; Vicente,W.R.R..
ABSTRACT The aim of this study was to determine the vascular indices of adrenal blood flow in healthy dogs (systolic velocity - SV; diastolic velocity - DV; resistance index - RI). Eighteen dogs (thirty six adrenal) were studied. Physical examination, biochemical profile and dexamethasone suppression test were performed to determine general health status. Echotexture, size, contours and margins, and overall shape of the adrenal gland (right and left) were assessed via ultrasound. By spectral Doppler of the phrenic-abdominal artery, the SV, DV, and RI were acquired. Animals did not show alterations in clinical and laboratory examination and suppression of cortisol. Normal homogeneous and echotexture, regular contours and margins and normal shape and size...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Adrenal glands; Ultrasound; Vascularization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400907
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Carcinoid of the gallbladder in two dogs Ciência Rural
Argenta,Fernando Froner; Pereira,Paula Reis; Bertolini,Marianna; Fratini,Letícia Mendes; Saccaro,Renata de Oliveira; Sonne,Luciana; Driemeier,David; Pavarini,Saulo Petinatti.
ABSTRACT: A carcinoid is a rare neoplasm derived from neuroendocrine cells that may arise in the hepatobiliary system. This report described two cases of carcinoid of the gallbladder in dogs, with emphasis on the clinical, pathological and immunohistochemical aspects. Case 1 was a 10-year-old female Shih Tzu, with a history of prostration and occasional episodes of diarrhea and vomiting. Case 2 was a 10-year-old male Cocker Spaniel, with a history of diarrhea and abdominal pain. Abdominal ultrasonography suggested a neoplasm of the gallbladder in both cases. The dogs underwent cholecystectomy, and 10% formalin-fixed samples were sent for pathological examination. Histologically, a neoplastic proliferation of neuroendocrine cells was observed in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Neoplasm; Neuroendocrine carcinoma; Immunohistochemistry.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200551
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Cervical and transdiaphragmatic paraxiphoid thoracoscopy in dogs: evaluation of respiratory response and blood pressure Ciência Rural
Guedes,Rogério Luizari; Feranti,João Pedro Scussel; Santos,Fabiane Reginatto dos; Brambatti,Gustavo; Tomazzoni,Fernando Variani; Oliveira,Marília Teresa de; Brun,Maurício Veloso; Souza,Fernando Wiecheteck de.
This study evaluated the cervical and transdiaphragmatic thoracoscopic approaches regarding their implementation, intrathoracic evaluation and application of a chest tube, using the treatment of pneumothorax as an experimental model. After injection of 20ml kg-1 of air per hemithorax, thoracoscopy was made by transdiaphragmatic paraxiphoid or cervical positions. After cavity examination, a thoracic drain was inserted using the surgical site to drain the pneumothorax. The cardiorespiratory function and arterial blood gas were measured during time of evaluation. The cavity examination allowed visualizing the aspect and function of intrathoracic structures. There were no significant differences between the two approaches when comparing the measurements made...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Chest tube; Pneumothorax; Video-surgery.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000701277
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Cloning and Characterization of a 2-Cys Peroxiredoxin from Babesia gibsoni OAK
MASATANI, Tatsunori; ASADA, Masahito; ICHIKAWA-SEKI, Madoka; USUI, Miho; TERKAWI, Mohamad A.; HAYASHI, Kei; KAWAZU, Shin-ichiro; XUAN, Xuenan; 河津, 信一郎; 玄, 学南.
Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni.
Palavras-chave: Antioxidant activity; Babesia gibsoni; Canine; Peroxiredoxin.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3925
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Consequences of intraoperative spinal cord manipulation in dogs with thoracolumbar intervertebral disc extrusion Arq. Bras. Med. Vet. Zootec.
Diogo,C.C.; Tudury,E.A.; Bonelli,M.A.; Araújo,B.M.; Figueiredo,M.L. de; Fernandes,T.H.T.; Silva,A.C.; Baraúna Júnior,D.; Santos,C.R.O.; Amorim,M.M.A.; Arias,M.V. Bahr.
ABSTRACT The objective of the present study was to evaluate if extradural contact during hemilaminectomy would cause neurological deterioration in the early and/or late postoperative period in dogs with intervertebral disc extrusion. Nineteen dogs with thoracolumbar intervertebral disc extrusion underwent hemilaminectomy for spinal cord decompression and removal of extruded disc material. Meningeal contacts during surgery were quantified. Paraplegia (with nociception) and paraparesis were observed in 11/19 and 8/19 of dogs, respectively, before surgery. At the end of our study, only two (2/19) had paraplegia and one (1/19), paraparesis. There were more extradural contacts when extruded intervertebral disc material was at a ventrolateral position....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Disc herniation; Hemilaminectomy; Spinal cord.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601473
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Cryopreservation of Canine Ovaries by Vitrification OAK
ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安.
The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23...
Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023
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Cutaneous mast cell tumor in dogs: a brief review MV&Z
Melo, Isadora Helena de Sousa; Magalhães, Geórgia Modé; Alves, Carlos Eduardo Fonseca; Calazans, Sabryna Gouveia.
In dogs, mast cell tumor is one of the most frequent malignancies, whose cutaneous presentation achieve levels of 11%. The present paper review the available literature of canine cutaneous mast cell tumor discussing it’s incidence, etiology, clinical presentation, clinical stage, diagnosis, treatment and prognosis. In spite of the actual advances in the diagnosis and treatment, of this pathology it’s prognosis is very uncertain because the clinical, biological behavior and treatment responses are so variable.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Mastocytoma; Review Canino; Mastocitoma; Revisão.
Ano: 2013 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/5373
Registros recuperados: 80
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